Mmi cellcut. See full list on img1.

Mmi cellcut. May 17, 2021 · The MMI CellCut is our Laser Microdissection system, designed to cut tissues, large regions of interest or single cells, and even living cells for further processing. The MMI CellCut enables an easy and gentle cutting of living cells to obtain a homogeneous live-cell population. pdf,MMI CellCut®Plus 激光显微切割系统培训手册基因有限公司技术支持部二零一九年八月目录 ® 一、 CellCut 激光显微切割系统技术特点二、系统安装条件三、培训所需试剂设备及样品四、安装及调试安排 Nov 20, 2012 · 瑞士MMI公司的单细胞获取平台有两大旗舰产品：CellEctor Plus单细胞分选系统和CellCut Plus激光显微切割系统。 • CellEctor Plus可从血液、骨髓、制备的组织细胞悬浮等液态体系中挑选出感兴趣的单细胞; Whole slide scanner with the highest flexibility CellScan is compatible with all objectives, numerous research microscopes and all possible imaging modes. 厂家简介 MMI公司成立于1998年的Switzerland，致力于将高精度的激光技术应用于生命科学领域，目前已经开发众多尖端产品。系统组成固态紫外激光器高精度XY 载物台倒置显微镜 CCD相机基于Windows系统的控制软件计算机性能特点 355 nm固态紫外激光器，寿命10,000 小时左右，免维护；高频（>5kHz Products Products 激光 “CapSure” 显微切割以分离组织中的细胞（MMI CellCut）基于毛细管的单细胞选择性分选（MMI CellEctor）激光光镊定量生物力（MMI CellManipulator）显微镜集成的全玻片 (细胞)成像（MMI CellScan）因此，第二条产品线已经开发出：采用高精度激光显微切割技术的产品，并于2001年推出了MMI CellCut。仅仅两年后，第三条产品线：采用毛细管毛细管细胞分选技术的仪器被引入。 The MMI CellCut system is elementary for our research because it enables us to select and investigate even single infected plant nuclei. In addition we use the MMI CellEctor to collect protoplasts from liquid media to generate new plants from a single cell. FIRST IN CUTTING-EDGE TECHNOLOGY The mmi CellCut system is the ultimate laser microdissection tool of resear-chers who effortlessy wanted to isolate groups of cells, single cells and cell components for analysis across a wide range of applications. 17img. MMI CellCut激光显微切割系统（LMD）专为细胞和组织的快速精确分离而设计，是分子病理学的必备工具。样品包括新鲜冷冻，石蜡包埋，存档载玻片，细胞涂片，涂片和活细胞在内，都可用于诊断目的。 Aug 3, 2023 · 基因有限公司的空间组学方案针对以上关键步骤，将MMI CellCut激光显微切割系统与Covaris AFA聚焦超声系统结合，推出了空间蛋白组学研究方案。 Jun 28, 2019 · In this study, we demonstrate how to easily use the MMI CellCut system, in combination with special cell-culture Membrane Slides, to isolate living cells directly into MMI Isolation Caps for Contamination-free Microdissection mmi® MembraneSlide® and a regular glass slide protect your sample from environmental contamination Designed for High-Modularity Integration The compact MMI optical tweezers is a modular system, which can be composed individually according to your needs and experimental requirements. The CellCut is contamination-free, reproducible and reliable and optimized for high-quality and quantity DNA, RNA, protein recovery. To change the settings and play the video, please click the button below and consent to use of "Functional" tracking technologies. See full list on img1. Arcturus also developed new instruments (Veritas™ and XT™) in which both pulsed IR and UV lasers can be used (Fig. This comprehensive presentation guides you through the workflow of Laser Microdissection and demonstrates unique features of the MMI CellCut, such as live cell cutting applications, the serial 通过直观的指向，点击和拖动程序来操纵多达20个微粒和活细胞创建0. Shop | MMI Webshop Our MMI CellCut combines several proven technologies which enable fast and precise laser microdissection of paraffin and cryo samples, smears and live cell cultures. 德国MMI（Molecular-Machines & Industries GmbH）专业研发CellCut激光显微切割（激光显微捕获）系统、CellEctor单细胞分选系统、CellManipulator激光光镊系统、CellScan高分辨率大视野玻片或细胞扫描成像系统。 The MMI CellCut system is elementary for our research because it enables us to select and investigate even single infected plant nuclei. 4. This gap does only exists in the legacy versions CellCut Plus and SmartCut Plus but is covered in CellCut systems starting from 2015. 5. Leica’s LMD7000 allows the cells to fall by gravity into a collection tube. MMI CellCut facilitates precise and contamination-free laser microdissection of cell clusters, single cells or subcellular compartments from various types of tissues including fresh frozen, paraffin embedded and archived slides, cytospins, smears and even living cells. Clinically related applications such as molecular pathology, microbiology, and virology will also find the mmi CellCut Plus of primary importance and value. The MMI CellCut is designed to facilitate laser microdissection from tissue sections, such as fresh frozen or FFPE material. For each LM target, sample collection was conducted minimum in duplicates. A plain glass histological slide is used as a cover for the tissue section and a support for the membrane during microdissection. A. 菲菲的vlog015｜和丽丽的毕业旅行｜长沙0731｜好吃到不想走的城市 mRNA表达的增加表示为红色阴影，而减少表示为蓝色阴影。（C）通过实时定量PCR证实了接受Thy11细胞移植的肝脏的SHPC和没有SHPC的肝细胞中IL17rb的表达。星号表示统计学上的显著差异， p ＜0. mmi CellCut Plus is ideal for scientists interested in the following areas of research: molecular/cell biology, genomics and proteomics, forensics, cancer and stem cell research. This advanced system offers seamless compatibility with the versatile ZEISS Axio Observer, a high-performance microscope platform with exceptional optical quality and flexibility. 6). The MMI CellCut was initially developed for the selective isolation of single cells or cell clusters from tissue sections using laser microdissection (Figure 1). Together with the MMI CellCut, CellEctor and CellDetector it enables new areas of applications, like single cell isolation and automated cell analysis. In addition we use the MMI CellEctor cell picker to collect protoplasts from liquid media to generate new plants from a single cell. The mmi CellCut Plus is ideal for scientists interested in the following areas of research: molecular or cell biology, genomics and proteomics, forensics, cancer and stem cell research. CellCut & CellEctor). ” MMI CellEctor CellCut 激光显微切割及单细胞分选系统工作站单细胞获取工作站，可以从石蜡、冰冻切片、新鲜组织切片、细胞悬液、处理过的外周血等多种不同类型的样本中获取以单细胞为单位的均质细胞或稀有细胞 Nov 18, 2024 · The MMI CellCut is known for its ability to perform highly gentle and contamination-free dissection, setting a new standard in single-cell analysis. And Zeiss’ PALM MicroBeam catapults the cells into the tube via a laser-induced pressure wave. For the attainment of valuable data from individual cells, precisely cut cell clusters, single cells, or even subcellular components, the MMI CellCut combines the most precise cutting performance and ensures Feb 1, 2018 · The MMI CellCut™ instrument (Molecular Machines & Industries, Germany) glues the microdissected cells on a sticky cap (Fig. It is compatible with all other MMI Single Cell Solutions (e. Sep 15, 2020 · The MMI CellCut Laser Microdissection system and the MMI CellScan Whole Slide Imaging system are both integrated on research microscopes and fully compatible with common objectives and imaging 德国Molecular Machines & Industries (MMI)是专门致力于将显微操作技术和高精度激光技术相结合应用于生命科学领域的一家专业公司，其开发的主要产品有MMI CellCut Plus激光显微切割系统和CellEctor Plus单细胞分选系统等。 MMI CellCut激光显微切割系统采用355 nm波长固态紫外激光器，激光脉冲时间短（皮秒级）且 The MMI CellCut is highly modular and can be mounted on numerous brands of microscope from entry level, mid range to high end, suitable for the most routine to the most complex research applications. The MMI Cell Tools are a fully modular instrumentation family, including the following components: - MMI CELLCUT® laser microdissection to isolate single cells or areas of tissue - MMI CELLMANIPULATOR® optical tweezing to manipulate cells or beads with an optical trap - MMI CELLECTOR® automated micro-pipetting to me- chanically manipulate ，MMI激光光镊--GFP，MMI CellCut 切割含有培养液的贴壁活细胞，MMI单细胞分选系统获取贴壁细胞团，80微米直径毛细针，MMI 激光显微切割--300微米厚新鲜小鼠脑片演示，MMI 激光显微切割--40微米厚小鼠膝关节切割演示，MMI 激光显微切割一键式高速质检，MMI 激光显微 The mmi CellTools are a fully modular instrumentation family, including the following components: • mmi CellCut: laser microdissection to isolate single cells or areas of tissue • mmi CellManipulator: optical tweezers to manipulate cells or beads with an optical trap • mmi CellEctor: automated micro-pipetting to mechanically manipulate cells or beads with a capillary and mechanical MMI CellCut 与经批准的 MMI膜皿（内皿）和 MMI分离皿（外皿）相结合，可以在活培养物中无污染地分离活细胞。可以避免酶处理（即胰蛋白酶）、其他潜在有害的选择性试剂以及耗时的重复富集。这样做的好处是提高了研究的可靠性、有效性和准确性。 •Laser access gap 1:Access to 10 mW UV laser power, gap between 90–97 mm, depends on the z-position of the objective and condenser •Laser access gap 2:access to 12 mW UV laser power, gap between 4–11 mm, depends on the z-position of the objective. ” Feb 2, 2024 · 本篇文章开发了基于MMI CellCut的激光显微空间可视化蛋白质组学平台，使得病理专家远程挑选目的细胞与LMD流程进行无缝对接。 Nov 4, 2024 · 德国 Molecular Machine & Industries (MMI) 是专门致力于将显微操作技术与高精度激光技术相结合应用于生命科学领域的一家专业公司，其标志性产品MMI CellCut激光显微切割系统和CellEctor单细胞分选系统成为目前世界上性能优异的样本处理系统。 Shop | MMI Webshop MMI instruments are an important basis for our in-situ analysis of cellular tissue. The MMI CellCut facilitates precise and contamination-free dissection of various types of tissues in a fully auditable and automatically documented workfl ow: MMI Cellcut Plus 显微切割系统,MMI产品介绍 CellCut Plus全自动激光显微切割系统可方便快捷地将不同类型的细胞从样本中分离下来，如肿瘤组织中的肿瘤细胞,获得单一类型的同质细胞，使后续的基因组、蛋白质组等水平的研究更加精细、准确。高精度的激光切割性能、“三明治”式的样本处理方法以及管 Summary Laser Microdissection serves as an essential foundation across various research domains, providing accurately defined initial material crucial for numerous subsequent experiments. The MMI CellScan is a whole slide scanner that captures full-resolution digital slides in 5D, including time-lapse, z-stack, XY scan, and multi-color fluorescence imaging. The MMI CellScan is compatible with a wide range of research microscopes and imaging modes, and can be combined with the MMI CellCut for laser microdissection. 用MMI CellCut激光显微切割系统收集BA组织中CTB-555+的神经元胞体大小的区域 (图3D，E)。对于每只小鼠，收集了500个这样的BA-NAC神经元样本，进行差异基因表达分析。 Jul 10, 2025 · MMI激光显微切割,激光捕获,显微捕获切割,MMI CellCut Plus 可以帮助您：以最高的收集率，安全、可靠、无污染的从组织切片中成功获取单细胞、染色体、均质细胞、细胞群，或者从贴壁培养的细胞中获取单细胞或细胞群。以供下游实验分析或再培养。产品特点：在 The mmi CellTools are a fully modular instrumentation family, including the following components: • mmi CellCut: laser microdissection to isolate single cells or areas of tissue • mmi CellManipulator: optical tweezers to manipulate cells or beads with an optical trap • mmi CellEctor: automated micro-pipetting to mechanically manipulate Jul 25, 2025 · The MMI (Molecular Machines & Industries ) CellCut facilitates precise and contamination-free dissection of cell clusters, single cells or subcellular compartments from various types of tissues including fresh frozen, paraffin embedded and archived slides, cytospins, smears and even living cells. 简介： MMI CellCut Plus将高精度紫外激光技术与高性能研究级别倒置显微镜相结合，采用三明治模型进行样本切片制备处理，在显微镜平台上使用紫外激光束将目标部分切割下来，用管盖粘附来收集目标样品，实现全程无污染，可监控的，快速的可靠的收集目标样本。主要特征： 1、采用奥林巴斯 The MMI CellCut Universal is a user-friendly laser microdissection tool to isolate groups of cells, single cells, and cell components for analysis across a wide range of applications. Jul 2, 2012 · MMI’s CellCut Plus uses an adhesive-coated collection cap that sticks to a membrane above the cells. 0。它具有新的界面样式，深色设计非常适合荧光应用。 MMI CellCut based on Axio Observer facilitates precise and contamination-free dissection of cell clusters, single cells or subcellular compartments from various types of tissues including fresh frozen, parafin-embedded and archived slides, cytospins, smears and even living cells. 3μm，因此可对目标区域进行超精确和快速切除；激光脉冲能量又非常低（1μJ），不会对细胞内的大分子如DNA、RNA和蛋白质等造成伤害。不论是应对细胞涂片，还是石蜡 Jan 1, 2012 · 3. High-performance whole slide scanner The entire imaging process is in your control 联系人苏黎, 刘云, 范馨莉生产厂家 MMI 邮箱型号 CellCut Plus & Scan Apr 9, 2024 · The groundbreaking CellCut high-precision laser microdissection system enables the dissection of tissue areas, single cells and subcellular structures with accuracy that is unmatched on the market. The MMI CellCut laser microdissection followed by gene expression analysis is complementary for further routine methods like conventional optical microscopy (fluorescence), in-situ hybridization, and immunohistochemistry. 5 UV Cutting Mode Microdissection for NanoString Analysis Using the mmi CellCut Plus ® The mmi CellCut Plus ® instrument is designed to dissect sections on PET metal framed slides. 7). Jun 21, 2013 · The MMI IsolationCap® was used for collection of dissected targets on MMI CellCut Plus. M. For the attainment of valuable data from individual cells, precisely cut cell clusters, single cells, or even subcellular components, the MMI CellCut combines the most precise cutting performance and ensures outstanding sample integrity with the highest flexibility and ease of use. 6 days ago · MMI Cellcut Plus活细胞激光显微切割系统Cellcut Plus不仅能获得组织，细胞，甚至获得的细胞能够进行培养。适合：动物和植物材料，对玻片固定的材料或者贴壁细胞都能操作，对福尔马林或者石蜡，冰冻切片都能有效处理。主要技术特点：采用专利精确冷激光切割，再采用专利膜粘贴选择性捕获 Mar 16, 2024 · MMI CellCutPlus 激光显微切割系统用户培训手册说明书. In this study, we demonstrate how to easily use the MMI CellCut system, in combination with special cell-culture Membrane Slides, to isolate living cells directly into MMI Isolation Caps for subsequent omics analysis. 2至> 800 pN的最强激光光阱，以测量细胞间和细胞内作用力通过自动校准进行力谱实验将您的MMI CellManipulator与MMI CellCut和/或CellEctor系统结合使用，以分离组织和悬浮液中的单细胞 Discover How ZEISS Microscopes and MMI CellCut Work Seamlessly Together The video player is blocked due to your cookie preferences. The system is operated by the MMI software. Hello and Welcome to MMI! In this series of video Tutorials we’d like to show you the basics of how to use the MMI CellCut to get good results for your furth The MMI CellCut Universal is a user-friendly laser microdissection tool to isolate groups of cells, single cells, and cell components for analysis across a wide range of applications. 德国Molecular Machines & Industries (MMI)是专门致力于将显微操作技术和高精度激光技术相结合应用于生命科学领域的一家专业公司，其开发的主要产品有MMI CellCut Plus激光显微切割系统和CellEctor Plus单细胞分选系统等。 MMI CellCut激光显微切割系统采用355 nm波长固态紫外激光器，激光脉冲时间短（皮秒级）且レーザーマイクロダイゼクションシステム MMI CellCut P1TDB1000003-1 細胞解析装置細胞濃縮・回収・前処理装置 / 細胞解析装置シングルセル解析 / お問い合わせお気に入りに入れる共有する比較リストに入れる质谱分析 MMI CellCut激光显微切割平台特点 1、三明治式样本制备，使样本被保护在膜片 (MembraneSlide）与载玻片之间，避免环境中的灰尘或其他杂质污染以及酶等对RNA或蛋白的破坏。同时使得样本在切割时不直接接触激光产生热损伤。 Summary Laser Microdissection serves as an essential foundation across various research domains, providing accurately defined initial material crucial for numerous subsequent experiments. The MMI CellCut Plus laser microdissection instrument, MMI CellEctor Plus single cell sorting solution, and MMI CellManipulator Plus optical tweezers combined in one system MMI CellCut 激光显微切割平台特点三明治式样本制备，使样本被保护在膜片 (MembraneSlide）与载玻片之间，避免环境中的灰尘或其他杂质污染以及酶等对RNA或蛋白的破坏。 The MMI CellCut Standard is a user-friendly laser microdissection tool to isolate groups of cells, single cells, and cell components for analysis across a wide range of applications. The mmi CellTools instrumentation family The mmi CellTools are a fully modular instrumentation family, including the following components: mmi CellCut: laser microdissection to isolate single cells or areas of tissue mmi CellManipulator: optical tweezers to manipulate cells or beads with an optical trap 2、将钥匙拧成水平状态,打开mmi CellTools软件,钥匙下方按钮闪烁黄灯,按下该按钮,等待激光启动; 3、将膜片放在载物台上,在软件右下方选择对应载玻片的位置。 4、 4 倍镜下调节画面清晰,将画面移动在空白处,点击菜单栏处的下拉菜单,选择adjust white 指示灯在进行切割时亮起，开机完成。（二）目标区域的切割 1、将三明治膜片放置在载物台上（膜片在上，玻璃载玻片在下），如下图： MMI CellCut®Plus 激光显微切割系统培训手册基因有限公司技术支持部二零一九年八月 f目录 FIRST IN CUTTING-EDGE TECHNOLOGY The mmi CellCut system is the ultimate laser microdissection tool of resear-chers who effortlessy wanted to isolate groups of cells, single cells and cell components for analysis across a wide range of applications. 此外，可以将MMI CellExplorer（用于自动细胞识别的软件）实施到MMI CellTools程序包中，以方便识别目标细胞，以便随后使用MMI CellCut或MMI CellEctor进行细胞分离。 MMI在2018年7月发布了最新版本的软件包MMI CellTools 5. g. Its functionality and proficiency have been validated in numerous publications using fresh frozen or FFPE material in various research areas such as oncology, pathology, immunology, forensics, and crop science 5-9. In contrast to systems, that use upright microscopes, the MMI laser comes from below. The MMI CellCut facilitates precise and contamination-free dissection of cell clusters, single cells or subcellular compartments from various types of tissues including fresh frozen, paraffin-embedded and archived slides, cytospins, smears and even living cells. The MMI CellCut laser microdissection for cell isolation followed by gene expression analysis is complementary to further routine methods like conventional optical microscopy (fluorescence), in-situ hybridization, and immunohistochemistry. Shop | MMI WebshopPrev 1 2 3 Next Nov 21, 2024 · 本项研究是2023年11月南方科技大学田瑞军教授团队在BioRxiv预印本上发表的文章，文中使用MMI CellCut激光显微切割系统切割胰腺导管腺癌肿瘤微环境中的不同细胞类型（邻近的正常腺泡细胞Acinar、小叶内管的胰腺上皮内瘤变PanIN和胰腺导管癌细胞PDAC），后进行空间 MMI CellScan 切片扫描系统,MMI·MMI CellScan可以通过扫描获得全尺寸的数字切片，可以注释你需要的区域，也可以用激光切割你需要的细胞，为您提供了所有的切片扫描的功能 ·使用显微镜的20x物镜和我们的高灵敏度CMOS相机，在不到75秒的时间内可以扫描15mmx15mm的区域，相当于10分钟可以扫描整张切片 webAtF AdminLoading Capillary based Cell-Sorting: The MMI CellEctor is an integrated instrumentation solution for the fully manual or automated recognition, aspiration and deposition of single cells in suspension onto a wide range of cell culture or molecular biology device. L. The MMI CellCut facilitates precise and contamination-free dissection of cell clusters, single cells or sub-cellular compartments from various types of tissues including fresh frozen, paraffin The MMI CellCut Laser Microdissection system and the MMI CellScan Whole Slide Imaging system are both integrated on research microscopes and fully compatible with common objectives and imaging modes. Shop | MMI WebshopPrev 1 2 Next MMI CellTools Software combined with MMI CellExplorer Software for automatic cell recognition in fl uorescence or bright fi eld Interactive LED pen display Expandability: MMI CellCut Plus for laser microdissection MMI CellManipulator Plus optical tweezers Range of imaging confi gurations (Fluorescence, DIC, Phase contrast) The MMI CellCut facilitates precise and contamination-free dissection of various types of tissues in a fully auditable and automatically documented workfl ow: 6 days ago · MMI CellCut激光显微切割系统采用355 nm波长固态紫外激光器，激光脉冲时间短（皮秒级）且激发频率高，切割线细腻平滑，切割宽度约0. 1 D mainly uses a solid-state UV laser with a wavelength of 355 nm, emitted briefly in short laser pulses (picosecond level) to maintain a high excitation frequency, which is essential for the successful isolation of the targeted area. For the attainment of valuable data from individual cells, precisely cut cell clusters, single cells, or even subcellular components, the MMI CellCut combines the most precise cutting performance and ensures MMI CellCut 全自动激光显微切割系统可精准地从异质性高的组织切片中以单细胞分辨率切割分离获得单细胞或单一类型的均质细胞，比如从肿瘤组织中切割获得肿瘤细胞，从脑组织中获得某一特定类型神经元等，使后续的基因组、转录组、蛋白质组等组学的研究更加精细、准确。高精度的激光切割性能我们的MMI CellCut结合了多种成熟技术，可对石蜡和冷冻样品，涂片和活细胞培养物进行快速，精确的激光显微切割。为了切割感兴趣的区域，Molecular Machines＆Industries使用了超精密固态紫外激光，同时保持了样品的形态并确保了用于后续下游分析的原材料的质量。简介： MMI CellCut Plus将高精度紫外激光技术与高性能研究级别倒置显微镜相结合，采用三明治模型进行样本切片制备处理，在显微镜平台上使用紫外激光束将目标部分切割下来，用管盖粘附来收集目标样品，实现全程无污染，可监控的，快速的可靠的收集目标样本。主要特征： 1、采用奥林巴斯一、 CellCut®激光显微切割系统技术特点 CellCut®激光显微切割(荧光)系统,是通过紫外激光切割需要分离的组织,然后通过有黏性的Eppendorf管盖进行收集,这样就可以将特定类型的细胞从组织切片上分离下来。它主要应用于石蜡、冰冻切片,细胞培养片,以及细胞涂片等各种样本的特定类型细胞的分离。它 3. The MMI CellCut Standard is a user-friendly laser microdissection tool to isolate groups of cells, single cells, and cell components for analysis across a wide range of applications. 0. UV Cutting Mode Microdissection for NanoString analysis using the mmi CellCut Plus® The mmi CellCut Plus® instrument is designed to dissect sections on PET (polyethylene terephthalate) metal framed slides. In July 2018, MMI launched the latest version of the software package, MMI CellTools 5. cn Jun 28, 2019 · In this study, we demonstrate how to easily use the MMI CellCut system, in combination with special cell-culture Membrane Slides, to isolate living cells directly into MMI Isolation Caps for subsequent omics analysis. ” The MMI CellCut facilitates precise and contamination-free dissection of cell clusters, single cells or sub-cellular compartments from various types of tissues including fresh frozen, paraffin embedded and archived slides, cytospins, smears and even living cells. and Acturus and is a robust and reliable tool for contamination-free isolation of single cells or small groups of cells. 德国 Molecular Machine & Industries (MMI) 是专门致力于将显微操作技术与高精度激光技术相结合应用于生命科学领域的一家专业公司，其标志性产品MMI CellCut激光显微切割系统和CellEctor单细胞分选系统成为目前世界上性能优异的样本处理系统。 Shop | MMI WebshopPrev 1 2 3 Next Sep 1, 2023 · The Cellcut system (MMI, Switzerland) shown in Fig. Developed by Molecular Machines & Industries (MMI), the CellCut high-precision laser microdissection system facilitates clean and precise dissection of almost any type of sample, from entire areas Jan 18, 2018 · The MMI CellCut plus laser-microdissection system from Molecular Machines & Industries (Eching; Germany) followed the pioneers from P. 05。三、MMI CellCut激光显微切割系统助力丙型肝炎病毒慢性感染与清除的 In addition, the MMI CellExplorer, a software toolbox for automated cell recognition, can be implemented into the MMI CellTools package to facilitate identification of target cells for subsequent isolation with the MMI CellCut or MMI CellEctor. bop ljnge rqhwotf vgtq accde jmoc twhosrm knydwgwz iro kuoy

26th Apr 2024